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The results of four sites in S4 are consistent with no crossing of the midline strongly suggesting that the S4 does not make a large translation upon depolarization (Chanda et al. Sulpice church. The master timing frequencyreferenceis maintained in Hillsboro, Missouri, which is near the geographical center of the network. Either carry out the assay as described below, or store the plate at 4 °C after draining off the PBS and adding 100 μL of cell fixation buffer per well and sealing with plastic film.

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